Beilstein J. Org. Chem.2011,7, 1030–1035, doi:10.3762/bjoc.7.117
-amino-4-methylcoumarin; p-nitroaniline; proteolyticsubstrate; selenocarboxylate/azide amidation; synthon; Introduction
Chromogenic and fluorogenic amino acid/peptide conjugates are widely used as substrates in enzyme assays for protease activity and specificity [1]. Proteolytic cleavage of the amino